matrigel collagen i mixture  (Fisher Scientific)

Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Cellular growth at different days (1, 3, 6) of organoid-derived intestinal epithelial cells on different substrates (APTMS, PEIGA, PDMS, plastic). The cells were cultured on different adhesion proteins: Laminin 111 (A), Laminin 511 (B), Collagen I and Matrigel (C), Collagen I and Laminin 511 (D) and in ENR medium. The data points represent the means and error bars the standard deviations. N = 3. (E) Growth dynamics showing representative images of organoid-derived intestinal epithelial cells, cultured in the microchannels as outline by the white dashed lines, of the alternative covering the largest area from (A–D) respectively (nuclei blue, actin green). Scale bar = 200 μm.

Article Snippet: The coating was performed with two types of pure human recombinant Laminins, 511 (BioLamina) and 111 (BioLamina), and with two Collagen-based mixtures, Collagen I and Matrigel (Corning), and Collagen I and Laminin 511.

Techniques: Derivative Assay, Cell Culture

Journal: RSC Advances

Article Title: Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices †

doi: 10.1039/d4ra08290g

Figure Lengend Snippet: Cellular growth at different days (1, 3, 6) of organoid-derived intestinal epithelial cells on different substrates (APTMS, PEIGA, PDMS, plastic). The cells were cultured on different adhesion proteins: Laminin 111 (A), Laminin 511 (B), Collagen I and Matrigel (C), Collagen I and Laminin 511 (D) in CV medium. The data points represent the means and error bars the standard deviations. N = 3. (E) Growth dynamics showing representative images of organoid-derived intestinal epithelial cells, cultured in the microchannels as outline by the white dashed lines, of the alternative covering the largest area from A–D respectively (nuclei blue, actin green). Scale bar = 200 μm.

Article Snippet: The coating was performed with two types of pure human recombinant Laminins, 511 (BioLamina) and 111 (BioLamina), and with two Collagen-based mixtures, Collagen I and Matrigel (Corning), and Collagen I and Laminin 511.

Techniques: Derivative Assay, Cell Culture

Journal: Kidney international

Article Title: Neutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo

doi: 10.1038/ki.2008.395

Figure Lengend Snippet: Cells were suspended in a 70:30 collagen:Matrigel mixture and grown for 5 days before quantitation of tubular or cystic structures. (a) Heterozygous PH cells formed mostly linear branching structures with less than 3% cystic structures. (b) Pkd1 null PN cells formed a mixture of cystic structures (arrows) as well as structures that were more linear in shape (arrowheads). (c) Quantitation of the percentage of cystic structures formed for each cell genotype; n=8 for PN (4 each for PN18 and PN24) and n=6 for PH (3 each for PH2 and PH3) cells, respectively, *P<0.001. (d) A total of 1×104 cells from each genotype were plated and cultured for 48 h in the presence of 10% FBS before trypsinization and counting; n=4 for each cell type, *P<0.005 vs the PH2 or PH3 cell lines. (e) Cells were cultured at equal densities and labeled with BrdU for 30 min. BrdU-positive cells were detected by FACS analysis as described; n=4 for each cell type, *P<0.002 vs the PH2 or PH3 cell lines. (f) Cells were cultured at equal densities and serum starved for 48 h before harvest and Annexin V staining and FACS analysis as described; n=3 separate experiments for each cell line, *P<0.01 vs the PH2 or PH3 cell lines.

Article Snippet: 22 Briefly, cells were trypsinized and an equal number (1.5×10 4 per ml) suspended in a 70:30 mixture of Collagen Type-I and Matrigel (BD Biosciences, San Jose, CA, USA).

Techniques: Quantitation Assay, Cell Culture, Labeling, Staining

Journal: Kidney international

Article Title: Neutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo

doi: 10.1038/ki.2008.395

Figure Lengend Snippet: (a, b) Pkd1 null cells were grown in a 70:30 collagen:Matrigel mixture for 5 days in the (a) absence or (b) presence of added NGAL (20 μg/ml). (c, d) Quantification of cyst area and volume from cells grown as in (a, b). Images were obtained from 10 random fields of three separate experiments. (c) Relative cyst area (calculated as mean pixels2 by outlining 76 cysts (36 from PN18 cells and 40 from PN24 cells) for each condition) and (d) cyst volume (calculated as pixels3 assuming a spherical shape) were determined using the Adobe Photoshop software. *P<0.001.

Article Snippet: 22 Briefly, cells were trypsinized and an equal number (1.5×10 4 per ml) suspended in a 70:30 mixture of Collagen Type-I and Matrigel (BD Biosciences, San Jose, CA, USA).

Techniques: Software